Genetic Variation of Isozyme Polyphenol Oxidase (PPO) Profiles in Different Varieties of Capsicum annuum L.

Genetic Variation of Isozyme Polyphenol Oxidase (PPO) Profiles in Different Varieties of Capsicum annuum L.

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The genus Capsicum commonly known as chilli pepper is a major spice crop and is of cosmopolitan in distribution.Native polyacrylamide gel DEEP IMMUNE LICORICE-FREE electrophoresis (Native PAGE) was used to study the polyphenol oxidase (PPO) isozyme variation in 21 varieties of Capsicum annuum L.A maximum of 4 PPO bands were scored in five varieties i.e., Ca14, Ca15, Ca16, Ca19 & Ca20, while the minimum (2 bands) was observed in Parts four varieties (Ca3, Ca10, Ca13 & Ca17).

15 pair wise combinations showed highest average per cent similarity (100%) and the UPGMA dendrogram represented low genetic diversity.The present study revealed that considerable intraspecific differences were found in the varieties.Thus the results obtained could be used in fingerprinting the genotypes.